Aethina Italy

Aethina tumida (Small Hive Beetle) notified in Calabria region, southern Italy
Franco Mutinelli National Reference Laboratory for beekeeping
Istituto Zooprofilattico Sperimentale delle Venezie Viale dell’Università, 10 35020 Legnaro (PD) E-mail: fmutinelli@izsvenezie.it Brussels, 19 September 2014
Aethina tumida Murray (1867)
Phylum Arthropoda Class Hexapoda Order Coleoptera Family Nitidulidae Indigenous to South Africa

Aethina tumida
Source of infestation: • Soil • Fruits • wood • Honey bees • Beekeeping materials
Life cycle: 27-79 days
Aethina tumida - Italy
5/09/2014: first report 3 weak nuclei affected by larvae and adults, no other colonies in the same site
locality Sovereto, Gioia Tauro municipality, Reggio Calabria province
5.09.2014: nuclei packed, killed with ethil acetate and stored at - 80°C 6.09.2014: soil treatment with chlorpiriphos 17.09.2014: soil further investigated, escavated and treated with deltamethrin
Calabria region
20 km and 100 km radius
First identification of both larvae and adults
Locality: Sovereto Municipality: Gioia Tauro Province: Reggio Calabria
Aethina tumida
Cronology
10.09.2014: samples of larvae and adults sent to NRL 11.09.2014: confirmation by morphological examination
12.09.2014: note of the Ministry of Health for the first actions
16-17.09.2014: confirmation by EURL based on morphological examination and PCR
Aethina tumida
17/09/2014: second outbreak in an apiary of 41 colonies Only adults were detected (25 as a total)
locality Gallina, S. Fernando municipality, Reggio Calabria province
18.09.2014: OIE notification 19.09.2014: Decree of Veterinary Services of Calabria region signed with measures directed at eradicating outbreaks and preventing further spreading
20 km radius 20 km radius 12.09.2014 17.09.2014
4 apiaries visited with negative results in a radius of 2 km
Aethina tumida
15-18.09.2014: NRL and EURL in the area of interest
Meeting with representatives of regional and local Veterinary Services Field inspection of the site of first detection Field inspection of 5 apiaries in a radius of 2 km 1 of these was positive (2nd case), the remaining 4 were negative
Aethina tumida
EU Regulation
Regulation (EC) n. 1398/2003 of the Commission, of 5 August 2003, Decision 2003/881/EC of the Commission, of 11 December 2003 Decision 2004/216/EC of the Commission, of 1 March 2004 Regulation (UE) N. 206/2010 of the Commission, of 12 March 2010
Aethina tumida
In Italy Aethina tumida is a notifiable disease (Order of the Ministry of Health 20.04.2004, prophylaxis of Aethina tumida and Tropilaelaps spp.)
Measured adopted by the Authority (Regional Order)
• Immediate notification of Aethina tumida • Restriction of any movement related to honey bee colonies (IN and OUT) • Census of apiaries and colonies • 20 km protection zone: • census of apiaries • visit of all apiaries • visit of the colonies on the basis of a 5% expected prevalence • destruction of the infested apiaries • if negative, traps should be applied • traceability of commercial and migratory movement in and out of the zone • Since the outbreaks are very close to the Gioia Tauro port, an investigation is in progress to trace movements of commodities from at risk areas (South Africa, USA, Australia); the port is not authorized to receive live animals
Measured adopted by the Authority
• 100 km surveillance zone: • census of apiaries • visit of random selected apiaries • visit of the colonies on the basis of a 2% expected prevalence • destruction of infested apiaries • if negative, traps should be applied • traceability of commercial and migratory movement in and out of the zone
Thank you for your attention